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Bioss
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Merck KGaA
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Beyotime
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Image Search Results
Journal: Journal of Cellular and Molecular Medicine
Article Title: BMSC s and miR‐124a ameliorated diabetic nephropathy via inhibiting notch signalling pathway
doi: 10.1111/jcmm.13747
Figure Lengend Snippet: The primer sequences in qRT‐PCR assay
Article Snippet: Next, immunoblotting was carried out using antibody to Pdx‐1 (rabbit anti‐ Pdx‐1 antibody, 1:250; Abcam), Pax‐6 (rabbit anti‐ Pax‐6 antibody, 1:250; Abcam), insulin‐1 (rabbit anti‐ insulin‐1 antibody, 1:250; Abcam), Ngn3 (rabbit anti‐Ngn3 antibody, 1:250; Abcam), GK (rabbit anti‐GK antibody, 1:250; Abcam), nephrin (rabbit anti‐nephrin antibody, 1:400; Abcam), podocin (rabbit anti‐podocin antibody, 1:250; Abcam), CD2AP (rabbit anti‐CD2AP antibody, 1:250; Abcam), caspase‐3 (rabbit anti‐caspase‐3 antibody, 1:400; Beyotime, Shanghai), Bax (rabbit anti‐Bax antibody, 1:400; Beyotime, Shanghai), Bcl‐2 (rabbit anti‐Bcl‐2 antibody, 1:400; Beyotime, Shanghai), Notch1 (rabbit anti‐Notch1 antibody, 1:400; Beyotime, Shanghai),
Techniques:
Journal: Journal of Cellular and Molecular Medicine
Article Title: BMSC s and miR‐124a ameliorated diabetic nephropathy via inhibiting notch signalling pathway
doi: 10.1111/jcmm.13747
Figure Lengend Snippet: BMSC s in combination with miR‐124a regulated Notch1, NICD , Hes1 and Delta expressions. Podocytes cells were treated with PBS (Con) and 40 mmol/L HG ( HG ) for 24 h and were then co‐cultured with BMSC s, which were transfected with mock (M/ HG ), miR‐124a mimics (m/ HG ) and miR‐124a inhibitors (i/ HG ) for 48 h, respectively. A, The mRNA expression levels of Notch1, NICD , Hes1 and Delta were measured by qRT ‐ PCR assay, respectively (** P < .01 vs Con; ^ P < .05 or ^^ P < .01 vs HG ; & P < .05 or && P < .01 m/ HG vs M/ HG ; ## P < .01 i/ HG vs M/ HG ). B, The protein expression levels of Notch1, NICD , Hes1 and Delta were detected by Western blot assay
Article Snippet: Next, immunoblotting was carried out using antibody to Pdx‐1 (rabbit anti‐ Pdx‐1 antibody, 1:250; Abcam), Pax‐6 (rabbit anti‐ Pax‐6 antibody, 1:250; Abcam), insulin‐1 (rabbit anti‐ insulin‐1 antibody, 1:250; Abcam), Ngn3 (rabbit anti‐Ngn3 antibody, 1:250; Abcam), GK (rabbit anti‐GK antibody, 1:250; Abcam), nephrin (rabbit anti‐nephrin antibody, 1:400; Abcam), podocin (rabbit anti‐podocin antibody, 1:250; Abcam), CD2AP (rabbit anti‐CD2AP antibody, 1:250; Abcam), caspase‐3 (rabbit anti‐caspase‐3 antibody, 1:400; Beyotime, Shanghai), Bax (rabbit anti‐Bax antibody, 1:400; Beyotime, Shanghai), Bcl‐2 (rabbit anti‐Bcl‐2 antibody, 1:400; Beyotime, Shanghai), Notch1 (rabbit anti‐Notch1 antibody, 1:400; Beyotime, Shanghai),
Techniques: Cell Culture, Transfection, Expressing, Quantitative RT-PCR, Western Blot